This fits a global model to a collection of ITC experiments collected in buffers of the same pH, but different ionization enthalpies.

global_connectors.NumProtons

This is useful for analyzing a binding reaction that involves the gain or loss of a proton. The measured enthalpy will have a binding component and an ionization component. These can be separated by performing ITC experiments using buffers with different ionization enthalpies.

## Scheme¶

$\Delta H_{obs,buffer} = \Delta H_{intrinsic} + \Delta H_{ionization,buffer} \times n_{proton},$

where $$\Delta H_{intrinsic}$$ is the buffer-independent binding enthalpy, $$\Delta H_{ionization,buffer}$$ is the buffer ionization enthalpy, and $$n_{proton}$$ is the number of protons gained or lost.

## Parameters¶

parameter variable parameter name class
association constant $$\Delta H_{intrinsic}$$ dH_intrinsic thermodynamic
binding enthalpy $$n_{proton}$$ num_protons thermodynamic

## Required data for each experiment¶

data variable parameter name
ioinzation enthalpy $$\Delta H_{ionization,buffer}$$ ionization_enthalpy