Extended Van’t Hoff

An extended Van’t Hoff analysis that assumes constant heat capacity.


Fits a collection of ITC experiments collected in identical buffer conditions, but at different temperatures. The temperature of each experiment is taken from the heats file. Allows extraction of the heat capacity, as well as the enthalpy and binding constant at a reference temperature.


\[\Delta H(T) = \Delta H_{ref} + \Delta C_{p}(T - T_{ref})\]
\[K = K(T_{ref})exp \Big ( \frac{-\Delta H_{ref}}{R} \Big (\frac{1}{T} - \frac{1}{T_{ref}} \Big ) + \frac{\Delta C_{p}}{R} \Big ( ln(T/T_{re}) + T/T_{ref} - 1 \Big ) \Big )\]

By performing experiments at a minimum of two temperatures, one can extract the heat capacity \(\Delta C_{p}\), the enthalpy at a reference temperture \(\Delta H_{ref}\) and the binding constant at a reference temperature \(K_{ref}\).


parameter variable parameter name class
association constant \(K_{ref}\) K thermodynamic
binding enthalpy \(\Delta H_{ref}\) dH thermodynamic
heat capacity \(\Delta C_{p}\) dCp thermodynamic

Required data for each experiment

data variable parameter name
temperature (K) \(T\) temperature